Journal: International journal of immunopathology and pharmacology

Article Title: CHO expressed recombinant human lactoferrin as an adjuvant for BCG

doi: 10.1177/0394632015599832

Figure Lengend Snippet: Lung weight index of Erdman MTB infected mice. All vaccinated and non-vaccinated mice were infected with Erdman MTB by aerosol at ~100 CFUs/mouse. At set time points, the whole lung was weighed and compared to the weight of the whole mouse to calculate lung weight index (LWI). (a) LWI of groups at day 7 post infection. (b) LWI of groups as a function of time post infection. Data are represented as mean, error bars are standard deviation. Data are analyzed by one-way ANOVA, and Tukey post-hoc tests are applied. *P <0.05; **P <0.01.

Article Snippet: At 12 weeks post boost, mice were aerosol challenged with Erdman strain of Mycobacterium tuberculosis (MTB) (TMC 107, ATCC 35801).

Techniques: Infection, Standard Deviation

Journal: International journal of immunopathology and pharmacology

Article Title: CHO expressed recombinant human lactoferrin as an adjuvant for BCG

doi: 10.1177/0394632015599832

Figure Lengend Snippet: Erdman bacterial load in lung, liver, and spleen tissues. All vaccinated and non-vaccinated mice were infected with Erdman MTB by aerosol at ~100 CFUs/mouse. Up to 19 weeks post infection, whole lung collected and homogenized in 1×PBS, serially diluted, and plated onto 7H11 agar plates. Data are represented as Log10 CFU/organ. Data are represented as box-and-whiskers: average, box min and max identify first and third quartiles, and the error bars define min and max of the dataset. Data are analyzed by one-way ANOVA, and Tukey post-hoc tests are applied. *P <0.05; **P <0.01; ***P <0.001.

Article Snippet: At 12 weeks post boost, mice were aerosol challenged with Erdman strain of Mycobacterium tuberculosis (MTB) (TMC 107, ATCC 35801).

Techniques: Infection

Journal: International journal of immunopathology and pharmacology

Article Title: CHO expressed recombinant human lactoferrin as an adjuvant for BCG

doi: 10.1177/0394632015599832

Figure Lengend Snippet: Pathology of lung tissue during Erdman MTB infection. All vaccinated and non-vaccinated mice were infected with Erdman MTB by aerosol at ~100 CFUs/mouse. The large left lobe lung was collected post infection at times indicated. Formalin fixed tissue was processed, sectioned, and stained with H&E. Images were visualized at 20×. Dark purple clusters represent granulomatous responses of lymphocytes surrounded by light pink designated foamy macrophage clusters; and dark pink monocytic infiltration without foamy cytoplasm.

Article Snippet: At 12 weeks post boost, mice were aerosol challenged with Erdman strain of Mycobacterium tuberculosis (MTB) (TMC 107, ATCC 35801).

Techniques: Infection, Staining

Journal: International journal of immunopathology and pharmacology

Article Title: CHO expressed recombinant human lactoferrin as an adjuvant for BCG

doi: 10.1177/0394632015599832

Figure Lengend Snippet: Percent lung occlusion of mice infected with Erdman MTB. Image from Figure 4 was analyzed by Image J. Percent occlusion was calculated by dividing the area of granulomas (lymphocytes, foamy macrophage, and other infiltrating leukocytes) by the area of the entire lung tissue section and multiplying by 100. Data are a compilation of three lung sections per mouse with five mice per group. No measureable granuloma structures were observed at 1 week post infection. (a) Data are represented as box-and-whiskers: average, box min and max identify first and third quartiles, and the error bars define min and max of the dataset. Data are analyzed by one-way ANOVA, and Tukey post-hoc tests are applied. *P <0.05; **P <0.01; ***P <0.001. (b) Time course of percent lung occlusion of each group up to 19 weeks post infection. Data are represented as mean, with error bar defined by standard deviation.

Article Snippet: At 12 weeks post boost, mice were aerosol challenged with Erdman strain of Mycobacterium tuberculosis (MTB) (TMC 107, ATCC 35801).

Techniques: Infection, Standard Deviation

Journal: International journal of immunopathology and pharmacology

Article Title: CHO expressed recombinant human lactoferrin as an adjuvant for BCG

doi: 10.1177/0394632015599832

Figure Lengend Snippet: Lung tissue global cytokine environment post infection. All vaccinated and non-vaccinated mice were infected with Erdman MTB by aerosol at ~100 CFUs/mouse. Up to 19 weeks post infection, lung section was collected and homogenized by pestle in DMEM 10% FBS. The homogenate was incubated at 37°C for 4 h. Supernatants were collected, filtered, and analyzed by ELISA. (a) One week post infection. (b) Four weeks post infection. (c) Ten weeks post infection. (d) Nineteen weeks post infection. Data are represented as box-and-whiskers: average, box min and max identify first and third quartiles, and the error bars define min and max of the dataset. Data are analyzed by one-way ANOVA, and Tukey post-hoc tests are applied. *P <0.05; **P <0.01; ***P <0.001.

Article Snippet: At 12 weeks post boost, mice were aerosol challenged with Erdman strain of Mycobacterium tuberculosis (MTB) (TMC 107, ATCC 35801).

Techniques: Infection, Incubation, Enzyme-linked Immunosorbent Assay